WebThe half-life of a protein is defined as the time it takes for the concentration of the radiolabeled target protein to be reduced by 50% relative to the level at the beginning of the chase. Another method to determine the turnover rate of a given protein is referred to as cycloheximide blocking. Protein synthesis is inhibited by cycloheximide ... WebMar 13, 2014 · RUNX1 had a shorter half-life in the presence of MLL-BP and an even shorter half-life in the presence of MLL-ENL, compared with the vector control (Figure 2A-B). ... protein synthesis inhibitor cycloheximide (CHX) was added to a final concentration of 10 μg/mL. Cells were then harvested at indicated time points (0, 3, and 6 hours) and …
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WebThe half-life of PHLPP1 was detected by CHX assay. The interaction between USP1 and PHLPP1 was examined by immunoprecipitation and GST pull-down assay. Results: Both USP1 and PHLPP1 are low expressed in lung cancer cells and tissues and silencing of USP1 by RNA interference significantly decreased the half-life of PHLPP1, which in turn ... WebApr 1, 2024 · The stability of all p16-HA variants was examined by a CHX chase assay. All p16-HA variants were degraded in the 6 h CHX chase period, but the Nt-acetylated [ME] and [SD] variants had a significantly longer half-life than the Nt-acetyl-free [PE], [AP] and [GY] variants (Fig. (Fig.4A–C). 4 A–C). Hence, p16 protein stability directly ... homiletica basica
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WebTo further determine whether this difference was caused by a difference in protein stability, we determined the protein half-life of M1-37T and M1-37A by treating transfected cells with the protein synthesis inhibitor cycloheximide (CHX) over a time course, followed by western blot analysis (Fig 3B). WebNov 21, 2014 · CHX were added to block cellular protein synthesis at different time points. (d) Quantification of dMyc stability to show extended dMyc half-life by dReg RNAi. The dMyc level at each time point is ... WebOct 4, 2024 · To assess whether TFIIH indeed becomes unstable in the absence of BRM, we determined the half-life of XPB in BRM-depleted U2OS cells after blocking protein … historical analysis essay example