Mycothiol s-conjugate amidase

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Web16 dec. 2014 · Mycothiol S-conjugate amidase (Mca) is a key enzyme involved in MSH-dependent detoxification, which was first isolated from the acid-fast bacterium … Web27 sep. 2003 · The mycothiol S-conjugate of rifamycin S was produced under physiologically relevant conditions and was shown to be a substrate for Mca in both …

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WebMycothiol (MSH) is a novel thiol comprised of N-acetylcysteine amide-linked to GlcN-alpha(1-1)-Ins. It is the major thiol in most actinomycetes and is produced at millimolar … WebEC 3.5.1.115 - Mycothiol S-conjugate amidase IntEnz view ENZYME view. XML. IntEnz Enzyme Nomenclature EC 3.5.1.115. Names. Accepted name: mycothiol S-conjugate amidase Other name: MCA Systematic name: mycothiol S-conjugate 1D-myo-inositol 2-amino-2-deoxy-α-D-glucopyranosyl-hydrolase Reaction. 36543 [IUBMB] H 2 O. Name … roth e roller

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Web16 jul. 2008 · MSH forms S-conjugates (MSR) with a wide range of toxins, Fig. 1b (Newton et al. 2000b), including the antibiotic rifamycin S (Steffek et al. 2003).The enzyme mycothiol S-conjugate amidase (Mca) cleaves such S-conjugates to produce a mercapturic acid, AcCySR, which can be excreted from the cell, and the pseudo-disaccharide GlcN-Ins … WebProtein target information for Mycothiol S-conjugate amidase (Mycobacterium tuberculosis H37Rv). Find diseases associated with this biological target and compounds tested … rother oss gmbh

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WebMycothiol is composed of one unit each of 1-D-glucosamine, 1-D-myo-inositol and N-acetyl cysteine. Novel enzymes involved in mycothiol biosynthesis, mycothiol-mediated … Web20 feb. 2003 · To this end, we have screened approximately 1500 extracts derived from marine plants and invertebrates and terrestrial fungi for their ability to inhibit a newly … rother opticsWebMycothiol is a small molecular weight thiol exclusive to actinomycetes, a subgroup of Gram-positive bacteria. Mycothiol is composed of one unit each of 1-D... rother ossola

"Web16 dec. 2014 · The present study focuses on the genetic and biochemical characterization of mycothiol S-conjugate amidase (Mca) of Corynebacterium … " - Mycothiol s-conjugate amidase

Mycothiol s-conjugate amidase

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Web1 jun. 2006 · The deacetylase, MshB, was the first enzyme of mycothiol biosynthesis to be identified and is related to mycothiol S-conjugate amidase (Mca), an enzyme that cleaves various S-conjugates of mycothiol (MSR; AcCySR-GlcN-Ins) to produce the mercapturic acid AcCySR and GlcN-Ins [9].In M. tuberculosis MshB is not essential for synthesis of … WebMycothiol conjugate amidase mca (mycothiol s-conjugate amidase); A mycothiol (MSH, N-acetyl-cysteinyl-glucosaminyl-inositol) S-conjugate amidase, it recycles conjugated MSH to the N-acetyl cysteine conjugate and the MSH precursor. Involved in MSH-dependent detoxification of a number of alkylating agents and antibiotics.

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Web1 apr. 2010 · N-Acetylglucosaminylinositol (GlcNAc-Ins)-deacetylase (MshB) and mycothiol-S-conjugate amidase (Mca), structurally related amidases present in mycobacteria and other Actinomycetes, are involved in the biosynthesis of mycothiol and in the detoxification of xenobiotics as their mycothiol-S-conjugates, respectively. WebMSH has a role in the removal of toxic compounds such as antibiotics, via the action of mycothiol S-conjugate amidase (Mca). MSH fuses with the toxic compound and then Mca breaks down the complex into glucosaminylinositol that is used to synthesise more MSH and a mercapturic acid, which is subsequently excreted from the cell ( Newton et al. , 2000b; …

Web1 jun. 2006 · Mycothiol (MSH, AcCys-GlcN-Ins) is the major low molecular weight thiol in actinomycetes and is essential for growth of Mycobacterium tuberculosis. MshB, the GlcNAc-Ins deacetylase, is a key enzyme in MSH biosynthesis. MshB from M. tuberculosis was cloned, expressed, purified, and its properties characterized. WebMycothiol is comprised of N-acetylcysteine (AcCys) amide linked to 1D-myo-inosityl 2-amino-2-deoxy-alpha-D-glucopyranoside (GlcN-Ins) and is the predominant thiol found in …

Web22 aug. 2008 · The mycothiol S -conjugate amidase activity (2.5 ± 0.1 nmol/min per mg protein) in Rhodococcus AD45 cell extracts was twice that of M. smegmatis (Newton et al. 2000 ). Thus, any mycothiol adducts in Rhodococcus AD45 would have been further metabolized to produce mercapturic acids (Newton et al. 2000 ). Web1 okt. 2000 · Mycothiol S-conjugate amidase (Mca) is a key enzyme involved in MSHdependent detoxification, which was first isolated from the acid-fast bacterium Mycobacterium smegmatis [7].

Web1 apr. 2007 · The primary role of mycothiol is to maintain the intracellular redox homeostasis. As such, it acts as an electron acceptor/donor and serves as a cofactor in detoxification reactions for alkylating agents, free radicals and xenobiotics.

Web21 okt. 2003 · The mycothiol S-conjugate of rifamycin S was produced under physiologically relevant conditions and was shown to be a substrate for Mca in both oxidized and reduced forms. Significant activity was also seen with the mycothiol S-conjugate of the antibiotic cerulenin as a substrate for Mca. Publication types Research Support, Non … rother optiker passauWeb20 mrt. 2008 · SCO4967 encodes an amidase (Mca) that recycles mycothiol in detoxification pathway In S. coelicolor, the mycothiol biosynthetic genes mshA, B, C and D have been identified, and a putative gene (SCO4967) for MSH S ‐conjugate amidase (Mca) has been predicted ( Park et al ., 2006 ). st peter\u0027s catholic church romeWeb21 okt. 2003 · The mycothiol S-conjugate of rifamycin S was produced under physiologically relevant conditions and was shown to be a substrate for Mca in … rother oststeiermarkWebEvaluation of 2 against mycothiol S-conjugate amidase from Mycobacterium tuberculosis reveals good specific activity (7500 nmol min(-)(1) mg-protein(-)(1), vs 14 200 for 1), indicating that 2 can serve as a starting point for antitubercular drug design. Skip to search form Skip to main content Skip to account menu rother osttirol südWeb16 dec. 2014 · The present study focuses on the genetic and biochemical characterization of mycothiol S-conjugate amidase (Mca) of Corynebacterium glutamicum. Recombinant C. glutamicum Mca was heterologously expressed in Escherichia coli … st peter\u0027s catholic church somerset pa 15501Web5 jun. 2015 · Mycothiol conjugate amidase (Mca) is a metalloamidase that catalyzes the cleavage of MS-conjugates to form a mercapturic acid, which is excreted from the … st. peter\u0027s catholic church slinger wiWeb27 sep. 2003 · Mycothiol S -conjugate amidase (Mca) cleaves the amide bond of mycothiol S -conjugates of a variety of alkylating agents and xenobiotics, producing GlcN-Ins and a mercapturic acid that can be excreted from the cell. Mca of Mycobacterium tuberculosis (Rv1082) was cloned and expressed as a soluble protein in Escherichia coli. rother otobo