Phospho ire1 antibody

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August undefined, 2024

WebMar 9, 2024 · IRE1α Antibody (B-12) is a mouse monoclonal IgG 1 κ IRE1α antibody, cited in 50 publications, provided at 200 µg/ml raised against amino acids 371-560 of IRE1α of … WebPhospho-IRE1-S724 Rabbit pAb (AP0878) Publications (11) Datasheet SDS. Tested applications: WB Reactivity: Human, Mouse, Rat. Western blot analysis of NIH/3T3, using …

Anti-IRE1 (phospho S724) antibody (ab48187) Abcam

WebLane 1: Mouse Intestinal lysate; Probed with IL-1R1 (Tyr496) Polyclonal Antibody, Unconjugated (bs-5394R) at 1:300 overnight at 4°C. Followed by incubation with … WebRabbit polyclonal antibody to IRE1 (phospho Ser724). (A258133) Buy rabbit polyclonal antibody to IRE1 (A258133). Applications: WB, ICC/IF and more. Reactivity: Human, … dutchess recliner

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Webthose that recognize nonphosphorylated IRE1. To characterize our anti–phospho-S729 antibody, we treated naive XBP1-proficient and -deficient B cells with LPS for a course of 72 h, immunopre-cipitated IRE1 using an anti–mouse IRE1 antibody, and showed that this antibody recognizes the 130-kD phospho-IRE1 protein WebIRE1 alpha (phospho Ser724) antibody Cat No. GTX130378 Datasheet File See all IRE1 alpha products APPLICATION Application Note *Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications. Calculated MW 110 kDa. ( Note ) Positive Control HeLa (100 nM Calyculin A, 30 min) Predict Reactivity WebTo characterize our anti–phospho-S729 antibody, we treated naive XBP1-proficient and -deficient B cells with LPS for a course of 72 h, immunoprecipitated IRE1 using an anti–mouse IRE1 antibody, and showed that this antibody recognizes the 130-kD phospho-IRE1 protein produced by 48-h and 72-h LPS-stimulated XBP1-deficient B cells . To ... crystal andrus

Perfluorooctanoic acid induces cell death in TM3 cells via the ER ...

IRE1 alpha [p Ser724] Antibody - BSA Free - Novus Biologicals

WebThermo Fisher Scientific's Phospho-IRE1 alpha (Ser724) Antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: EIA, Immunoassay, Assay, ChIP, ELISA, Functional Assay, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry - fixed, Immunohistochemistry - frozen, Immunoprecipitation, and … Webphospho ire1 alpha phospho-ire1 alpha p-ire1 alpha Background Unfolded protein response (UPR) signaling is a mechanism used by eukaryotic cells to deal with endoplasmic reticulum/ER stress: an accumulation of unfolded proteins in the ER lumen. dutchess new york mapWebProduct name Anti-IRE1 (phospho S724) antibody [EPR23107-16] See all IRE1 primary antibodies Description Rabbit monoclonal [EPR23107-16] to IRE1 (phospho S724) Host species Rabbit Tested applications Suitable … dutchess park lake

"WebAnti-IRE1α antibody produced in rabbit (Anti-Endoplasmic reticulum to nucleus signaling 1 ); ~ 1mg/mL, affinity isolated antibody, buffered aqueous solution; Suitable for western blot; Yale Center for High Throughput Cell Biology IF-tested antibodies; Anti-IRE1α antibody produced in rabbit has USEN ApplicationsProductsServicesSupport I6785 " - Phospho ire1 antibody

Phospho ire1 antibody

ProductDataSheet FullSize v4 - Novus Bio

WebAug 23, 2024 · HEC-1-A cells were infected with HSV-1 (strain HF) at multiplicity of infection (moi) = 1. (A) Total cellular protein was harvested at indicated time point p.i. (post infection) and subject to Western blot analysis using antibodies against total IRE1, gD-1 (Glycoprotein D) and GAPDH (glyceraldehyde 3-phosphate dehydrogenase). The number under ...

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WebIRE1 alpha is essential for viability under stress conditions that cause unfolded proteins to accumulate in the ER. IRE1 alpha is a transmembrane protein that has both serine-threonine kinase and endoribonuclease activities and has a theoretical molecular weight of 110 kDa. WebThe protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity …

WebOur Anti-IRE1 (Ser724) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse IRE1 (Ser724) and is antigen affinity purified from pooled serum. It is great for use in WB. Western blot of mouse whole brain lysate showing specific labeling of the ~130 kDa IRE1 protein phosphorylated at ... WebPhospho-IRE1 alpha (Ser724) Polyclonal Antibody Advanced Verification This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. 29 Published Figures 63 References View all (42) IRE1 alpha antibodies Catalog # PA1 …

WebMay 7, 2024 · We generated an anti-phospho-S729 antibody to investigate such phosphorylation. Compared with pharmacological ER stress inducers or Toll-like receptor ligands, the bacterial subtilase cytotoxin has an unusual capability in causing rapid and strong phosphorylation at S729 and triggering B cells to express spliced XBP1. WebIRE1 Antibody detects endogenous levels of total IRE1. RRID: AB_2841178 Cite Format: Affinity Biosciences Cat# DF7709, RRID:AB_2841178. Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific). Storage:

WebAnti-IRE1 (phospho S724) antibody [EPR23107-16] - BSA and Azide free See all IRE1 primary antibodies Description Rabbit monoclonal [EPR23107-16] to IRE1 (phospho S724) - BSA and Azide free Host species Rabbit Tested …

WebThe antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns. Storage: … dutchess regional chamberWebGet better batch-to-batch reproducibility with a recombinant antibody. Anti-IRE1 (phospho S724) antibody [EPR5253] (ab124945) Research with confidence – consistent and … crystal andrus morissetteWebHowever, accumulation of unfolded proteins results in dissociation of BIP from IRE1 and oligomerization of IRE1 accompanied by the upregulation of GRP78/BIP which is consistent with our results [53]. Activation of IRE1 initiates the recruitment of the TRAF2/ASK1 complex and activate the Jun-N-terminal kinase (JNK), one of the stress kinases [54]. dutchess storm photosWebAnti-IRE1 (phospho S724) antibody [1F12] See all IRE1 primary antibodies Description Rabbit monoclonal [1F12] to IRE1 (phospho S724) Host species Rabbit Tested applications Suitable for: ICC, WB more details Species … dutchess songWebDescription. IRE1 alpha is the endoplasmic reticulum to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. IRE1 alpha protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals. crystal angel christmas ornamentsWebAnti-IRE1 alpha (phospho Ser724) antibody (GTX132808) GeneTex Back Primary Antibodies Secondary Antibodies Antibody Panels ELISA Antibody Pairs & Kits Isotype Controls Proteins & Peptides Slides Lysates Serums & Plasmas Reagents Research Kits Research Tools Back Cancer Tumor Markers Neuroscience Cell Biology Metabolism … dutchess spca catsWebSeparation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody. Cell Counting Kit-8 (CCK-8) A convenient and sensitive way for cell proliferation assay and cytotoxicity assay. SYBR Safe DNA Gel Stain Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels. ... dutchess silver tree deep creek