Pyt313
WebFeb 2, 2024 · These two fragments were then cloned into the Bam HI and Sph I sites of suicide vector pYT313, using Gibson Assembly . Resulting plasmids were moved into F. johnsoniae, via tri-parental mating , and erythromycin (Em, 100 μg/mL) resistant transconjugants were selected. WebDec 13, 2024 · Mutations to the F. johnsoniae chromosome were made using precise allelic replacement (Zhu et al., 2024).Alleles were cloned into the Bam HI and Sph I restriction sites of the suicide vector pYT313 (Zhu et al., 2024).This vector has two selectable markers, bla (expressed in E. coli) and ermF (expressed in F. johnsoniae), as well as the counter …
Pyt313
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WebJan 11, 2024 · The vector pYT313 (ref. 38) was linearized by amplification with primers FL313 and FL314. These three fragments were then assembled by Gibson cloning to … WebFeb 21, 2024 · The first step is to insert two DNA fragments upstream and downstream of porY into pYT313. Specific Content: For the past few weeks, we had conducted plasmid preparation from the Escherichia coli bacterium, otherwise known as E. coli, polymerase chain reaction (PCR), gel electrophoresis, purification, and digestion.
WebAug 30, 1995 · With multiple (M) mutagenesis, TaG4GSG~'CTGs to A3C4GSC6GTC8 at the right end (pYT315), transpositions are not detected. Interestingly, if both ends are altered to restore an inverted repeat (pYT313), a low but reproduc- ible level of transposition is detected. How can the double mutant rescue be explained'? WebJan 26, 2024 · Briefly, two 1- to 1.6-kb regions flanking each gene were cloned into plasmid pYT313 using the HiFi assembly kit (New England Biosciences). Sequence integrity was …
WebFeb 21, 2024 · The first step is to insert two DNA fragments upstream and downstream of porY into pYT313. Specific Content: For the past few weeks, we had conducted plasmid … WebComprehension of the degradation of macroalgal polysaccharides suffers from the lack of genetic tools for model marine bacteria, despite their importance for coastal ecosystem functions. We developed such tools for Zobellia galactanivorans, an
WebCells of Flavobacterium johnsoniae exhibit rapid gliding motility over surfaces. Cell movement is thought to involve motor complexes comprised of Gld proteins that propel …
WebShine-Dalgarno sequences (SD) in prokaryotic mRNA facilitate protein translation by pairing with rRNA in ribosomes. Although conventionally defined as AG-rich motifs, recent … buchanan connectorsWebCells of Flavobacterium johnsoniae move rapidly over surfaces by a process known as gliding motility. Gld proteins are thought to comprise the motor that propels the cell … extended forecast for michiganWebMap of the sacB-containing suicide vector pYT313 used for constructing chromosomal gene deletions. pYT313 was constructed by introducing sacB and the promoter of F. … extended forecast for milton flWebFeb 15, 2024 · Comprehension of the degradation of macroalgal polysaccharides suffers from the lack of genetic tools for model marine bacteria, despite their importance for … extended forecast for myrtle beach sc 10 daysWebFeb 14, 2024 · The A. machipongonensis AlgoCIS deficient mutants generated in this paper are all site-directed insertion mutants with pYT313 66, a bacteroidetes suicide plasmid … extended forecast for maynardville tnWeb(A) ompA promoter (pOmpA) was amplified from pYT313, PCR-mutagenized, and inserted into the pCP11 plasmid in front of the actA or actE gene, generating a series of plasmids … buchanan consultingWebA. Map of the sacB-containing suicide vector pYT313 used for constructing chromosomal gene deletions. pYT313 was constructed by introducing sacB and the promoter of F. … buchanan contracting group